ABSTRACT
Objective To find out the relationship between fetal growth restriction (FGR) and transforming growth factor -β1 (TGF-β1). Methods The levels of TGF-β1 in maternal serum and placental tissue of FGR were detected with using ELISA and immunohistochemistry technique, and it was compared with those of normal term pregnancy. Results The levels of TGF-β1 in maternal serum of FGR group were significantly higher than those of normal term pregnancy (76. 5 ± 33. 4 VS 47.6 ± 24. 2, t' = 4. 65, P ＜0. 05). As for the intensity of the immunohistochemistry signal, TGF-β1 in syncytiotrophoblastic cells of FGR was markedly higher than that of control group (81.82% vs 5.83%, P ＜0. 01). Conclusions The levels of TGF-β1 are closely related with FGR.
ABSTRACT
Objective To investigate the different expression of NF-κB p65 Protein in placental tissues between premature delivery and term delivery and to explore the significance of nuclear factor kB (NF-κB) protein expression in preterm delivery. Methods Fifty premature delivery pregnant women and thirty term delivery pregnant women ( were enrolled in this study. According to the way of delivery , the patients were divided into two groups. The expression of NF-κB p65 protein was analyzed by using immunohistochemistry method in the placental tissues. Results In the term delivery, NF-κB p65 was mainly negative or weak positive expressed in the cytoplasm of cells in the placental tissues. There was no demonstrable difference in immunostaining of the NF-kB p65 subunit in the placental tissues including chorion, umbilical cord and fetal membranes before and after labor ( P ＞ 0.05 ). In the premature delivery, NF-κB p65 was strongly expressed in the cytoplasm and nuclear of cells. The expression of NF-κB p65 protein in chorion and fetal membranes from premature delivery( 18% ,56% ) was significantly higher than that from normal pregnant women (6.7% ,13.3%, P ＜0.01 ). But no significant change was found for NF-κB p65 expression in umbilical cord in pregnant women with or without premature delivery ( P ＞ 0.05 ). And in fetal membranes and deciduas, there was a significant increase in the staining of immunoreactive NF-κB p65 in preterm(52.9% ,58.8% ) by caesarean section compared to tissues obtained from term delivery( 13.3%,6.7%, P ＜0.01 ). In chorion and fetal membranes, there was significant increases in the staining of immunoreactive NF-κB p65 in preterm ( 24.2%, 57.6% ) by vaginal dehvery compared to tissues obtained term delivery (6.7%, 13.3%, P ＜0.01 ). Conclusion In this study, the expression of NF-κB p65 did not show significant change in term delivery before and after labor. NF-κB p65 in premature delivery was higher than term delivery, and it had no relationship with the delivery ways.